Ultra-rapid quantitative isolation of specific transfer ribonucleic acids a solid-phase method

D. J. Goss; L. J. Parkhurst

doi:10.1016/S0006-291X(74)80191-9

Ultra-rapid quantitative isolation of specific transfer ribonucleic acids a solid-phase method

D. J. Goss, L. J. Parkhurst

Research output: Contribution to journal › Article › peer-review

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Abstract

The following procedure is used in a rapid isolation of a specific (isoacceptor) tRNA from a mixture of deacylated tRNAs: (1) Acylation with the amino acid corresponding to the desired specific tRNA (2) Reaction of the specifically acylated tRNA with a new bi-functional reagent (PAMBSYL) (3) Binding of the pambsylated tRNA to a sulfhydryl-containing resin (4) Release of the specific tRNA from the resin after removal of the non-reacted tRNA. Steps 3 and 4 can be carried out in 20 min. One ml of the resin can bind approximately 200 mg of reacted tRNA.

Original language	English (US)
Pages (from-to)	181-187
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	59
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(74)80191-9
State	Published - Jul 10 1974

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(74)80191-9

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title = "Ultra-rapid quantitative isolation of specific transfer ribonucleic acids a solid-phase method",

abstract = "The following procedure is used in a rapid isolation of a specific (isoacceptor) tRNA from a mixture of deacylated tRNAs: (1) Acylation with the amino acid corresponding to the desired specific tRNA (2) Reaction of the specifically acylated tRNA with a new bi-functional reagent (PAMBSYL) (3) Binding of the pambsylated tRNA to a sulfhydryl-containing resin (4) Release of the specific tRNA from the resin after removal of the non-reacted tRNA. Steps 3 and 4 can be carried out in 20 min. One ml of the resin can bind approximately 200 mg of reacted tRNA.",

author = "Goss, {D. J.} and Parkhurst, {L. J.}",

note = "Funding Information: Acknowledgements. This work was supported by grants from Research Council of the University of Nebraska, a Nebraska Heart Association Grant-in-Aid, and NIH Grant No. HL 15284-02. We wish to thank Prof. Fred Wagner for providing samples of resin (I) and Profs. R. Johnston and J. van Etten for samples of (14C) amino acids for the assays of aeceptor activity.",

year = "1974",

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doi = "10.1016/S0006-291X(74)80191-9",

language = "English (US)",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Ultra-rapid quantitative isolation of specific transfer ribonucleic acids a solid-phase method

AU - Goss, D. J.

AU - Parkhurst, L. J.

N1 - Funding Information: Acknowledgements. This work was supported by grants from Research Council of the University of Nebraska, a Nebraska Heart Association Grant-in-Aid, and NIH Grant No. HL 15284-02. We wish to thank Prof. Fred Wagner for providing samples of resin (I) and Profs. R. Johnston and J. van Etten for samples of (14C) amino acids for the assays of aeceptor activity.

PY - 1974/7/10

Y1 - 1974/7/10

N2 - The following procedure is used in a rapid isolation of a specific (isoacceptor) tRNA from a mixture of deacylated tRNAs: (1) Acylation with the amino acid corresponding to the desired specific tRNA (2) Reaction of the specifically acylated tRNA with a new bi-functional reagent (PAMBSYL) (3) Binding of the pambsylated tRNA to a sulfhydryl-containing resin (4) Release of the specific tRNA from the resin after removal of the non-reacted tRNA. Steps 3 and 4 can be carried out in 20 min. One ml of the resin can bind approximately 200 mg of reacted tRNA.

AB - The following procedure is used in a rapid isolation of a specific (isoacceptor) tRNA from a mixture of deacylated tRNAs: (1) Acylation with the amino acid corresponding to the desired specific tRNA (2) Reaction of the specifically acylated tRNA with a new bi-functional reagent (PAMBSYL) (3) Binding of the pambsylated tRNA to a sulfhydryl-containing resin (4) Release of the specific tRNA from the resin after removal of the non-reacted tRNA. Steps 3 and 4 can be carried out in 20 min. One ml of the resin can bind approximately 200 mg of reacted tRNA.

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Ultra-rapid quantitative isolation of specific transfer ribonucleic acids a solid-phase method

Abstract

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