Objective: To investigate the enhancement of ultrasound thrombolysis in vitro using transcatheter injection of microbubble. Methods: Fresh human blood clots (n=40) divided into experimental group 1 (n=10, transcatheter injection of microbubble and urokinase+ultrasound), experimental group 2 (n=10, peripheral circulation injection of urokinase and microbubbles+ultrasound), control group 1 (n=10, transcatheter injection of microbubble and urokinase), control group 2 (n=10, peripheral circulation injection of urokinase and microbubbles). Therapeutic US was operated at the frequency of 1.0 MHz with the peak negative pressure of 512 kPa. The thrombolysis rate of clot was calculated after treatment. US release of MBDiO was observed fluorescent distribution and intensity in thrombus after treatment. Results: The thrombolysis rate of experimental group 1 (137.70%±3.17%) was significantly higher than that of experimental group 2 (11.67%±1.13%), control group 1 (14.37%±2.22%) and control group 2 (7.90%±0.68%, all P<0.05). Transcatheter injection of microbubble usually developed clusters of strong echo in thrombus with acoustic shadow. After treatment, the echo was scattered and spread. Laser confocal microscopy found that a large amount of bright fluorescent particles in the clots in experimental group 1, but rare fluorescence could be found in the clots in experimental group 2. Conclusion: The thrombolysis rate of transcatheter injection of microbubble and urokinase+ultrasound can significantly higher than the method of peripheral circulation injection of urokinase and microbubbles.
|Original language||English (US)|
|Number of pages||5|
|Journal||Chinese Journal of Interventional Imaging and Therapy|
|State||Published - Mar 10 2015|
- Transcatheter injection of microbubbles
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging