Ultrastructural heterogeneity of the alveolar macrophages from tobacco smokers with chronic bronchitis

V. V. Polosukhin; L. S. Manouilova; D. J. Romberger; K. I. Matthews; S. J. Pirruccello; W. West; D. M. Daughton; T. Millatmal; T. Umino; S. I. Rennard

doi:10.1080/019131201300004636

Ultrastructural heterogeneity of the alveolar macrophages from tobacco smokers with chronic bronchitis

V. V. Polosukhin, L. S. Manouilova, D. J. Romberger, K. I. Matthews, S. J. Pirruccello, W. West, D. M. Daughton, T. Millatmal, T. Umino, S. I. Rennard

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6 Scopus citations

Abstract

Alveolar macrophages recovered by bronchoalveolar lavage from 14 heavy smokers with chronic bronchitis were assessed. Ultrastructural examination revealed marked cellular heterogeneity. Three subpopulations of alveolar macrophages were readily identifiable. These have been termed "young," "mature," and "degrading," reflecting their ultrastructural features. In addition, a majority of the cells were found to be positive by TUNEL staining, indicating DNA damage, but a very small percentage tested positive for Caspase-3, suggesting that apoptosis might not account for the DNA damage in at least some of these cells. A small percentage of proliferating cells were noted.

Original language	English (US)
Pages (from-to)	5-11
Number of pages	7
Journal	Ultrastructural Pathology
Volume	25
Issue number	1
DOIs	https://doi.org/10.1080/019131201300004636
State	Published - 2001

Keywords

Alveolar macrophage
Apoptosis
Bronchoalveolar lavage
Chronic bronchitis
Proliferation
Ultrastructure

ASJC Scopus subject areas

Pathology and Forensic Medicine
Structural Biology

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10.1080/019131201300004636

Cite this

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title = "Ultrastructural heterogeneity of the alveolar macrophages from tobacco smokers with chronic bronchitis",

abstract = "Alveolar macrophages recovered by bronchoalveolar lavage from 14 heavy smokers with chronic bronchitis were assessed. Ultrastructural examination revealed marked cellular heterogeneity. Three subpopulations of alveolar macrophages were readily identifiable. These have been termed {"}young,{"} {"}mature,{"} and {"}degrading,{"} reflecting their ultrastructural features. In addition, a majority of the cells were found to be positive by TUNEL staining, indicating DNA damage, but a very small percentage tested positive for Caspase-3, suggesting that apoptosis might not account for the DNA damage in at least some of these cells. A small percentage of proliferating cells were noted.",

keywords = "Alveolar macrophage, Apoptosis, Bronchoalveolar lavage, Chronic bronchitis, Proliferation, Ultrastructure",

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year = "2001",

doi = "10.1080/019131201300004636",

language = "English (US)",

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T1 - Ultrastructural heterogeneity of the alveolar macrophages from tobacco smokers with chronic bronchitis

AU - Polosukhin, V. V.

AU - Manouilova, L. S.

AU - Romberger, D. J.

AU - Matthews, K. I.

AU - Pirruccello, S. J.

AU - West, W.

AU - Daughton, D. M.

AU - Millatmal, T.

AU - Umino, T.

AU - Rennard, S. I.

PY - 2001

Y1 - 2001

N2 - Alveolar macrophages recovered by bronchoalveolar lavage from 14 heavy smokers with chronic bronchitis were assessed. Ultrastructural examination revealed marked cellular heterogeneity. Three subpopulations of alveolar macrophages were readily identifiable. These have been termed "young," "mature," and "degrading," reflecting their ultrastructural features. In addition, a majority of the cells were found to be positive by TUNEL staining, indicating DNA damage, but a very small percentage tested positive for Caspase-3, suggesting that apoptosis might not account for the DNA damage in at least some of these cells. A small percentage of proliferating cells were noted.

AB - Alveolar macrophages recovered by bronchoalveolar lavage from 14 heavy smokers with chronic bronchitis were assessed. Ultrastructural examination revealed marked cellular heterogeneity. Three subpopulations of alveolar macrophages were readily identifiable. These have been termed "young," "mature," and "degrading," reflecting their ultrastructural features. In addition, a majority of the cells were found to be positive by TUNEL staining, indicating DNA damage, but a very small percentage tested positive for Caspase-3, suggesting that apoptosis might not account for the DNA damage in at least some of these cells. A small percentage of proliferating cells were noted.

KW - Alveolar macrophage

KW - Apoptosis

KW - Bronchoalveolar lavage

KW - Chronic bronchitis

KW - Proliferation

KW - Ultrastructure

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Ultrastructural heterogeneity of the alveolar macrophages from tobacco smokers with chronic bronchitis

Abstract

Keywords

ASJC Scopus subject areas

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