TY - JOUR
T1 - Unconjugated bilirubin inhibits in vitro cytotoxic T lymphocyte activity of human lymphocytes
AU - Haga, Yoshio
AU - Tempero, Margaret A.
AU - Zetterman, Rowen K.
PY - 1996/10/7
Y1 - 1996/10/7
N2 - Septic complications have been major problems in the management of patients with obstructive jaundice and neonatal jaundice. This study investigates effects of bilirubin on human T lymphocyte responses against allogeneic mixed lymphocyte reaction. In vitro exposure of human peripheral blood mononuclear cells (PBMNC) with unconjugated bilirubin at pathological levels (6 to 12 mg/dl) did not alter the subsets of CD3, CD4, CD8, CD14, CD19 and CD56 positive populations, or expression of costimulatory surface molecules CD2, CD3, CD4 and CD8, Further incubation of bilirubin-treated PBMNC with irradiated B lymphoid Raji cells after removal of the extracellular bilirubin resulted in a dose-dependent decrease of cytotoxic T lymphocyte (CTL) activity, DNA synthesis, and expression of Tac antigen (CD25) and transferrin receptor (CD71). However, no significant change of interleukin-2 (IL-2) production was observed after this incubation between bilirubin-treated and -untreated PBMNC. These results suggest that bilirubin inhibits the induction of CTL activity, and this defect may result from the impaired responsiveness against IL-2. These observations may help explain the increased infection observed in hyperbilirubinemic patients.
AB - Septic complications have been major problems in the management of patients with obstructive jaundice and neonatal jaundice. This study investigates effects of bilirubin on human T lymphocyte responses against allogeneic mixed lymphocyte reaction. In vitro exposure of human peripheral blood mononuclear cells (PBMNC) with unconjugated bilirubin at pathological levels (6 to 12 mg/dl) did not alter the subsets of CD3, CD4, CD8, CD14, CD19 and CD56 positive populations, or expression of costimulatory surface molecules CD2, CD3, CD4 and CD8, Further incubation of bilirubin-treated PBMNC with irradiated B lymphoid Raji cells after removal of the extracellular bilirubin resulted in a dose-dependent decrease of cytotoxic T lymphocyte (CTL) activity, DNA synthesis, and expression of Tac antigen (CD25) and transferrin receptor (CD71). However, no significant change of interleukin-2 (IL-2) production was observed after this incubation between bilirubin-treated and -untreated PBMNC. These results suggest that bilirubin inhibits the induction of CTL activity, and this defect may result from the impaired responsiveness against IL-2. These observations may help explain the increased infection observed in hyperbilirubinemic patients.
KW - Bilirubin
KW - Cytotoxicity
KW - Major histocompatibility complex
KW - T lymphocyte
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UR - http://www.scopus.com/inward/citedby.url?scp=0030573972&partnerID=8YFLogxK
U2 - 10.1016/0925-4439(96)00039-7
DO - 10.1016/0925-4439(96)00039-7
M3 - Article
C2 - 8876628
AN - SCOPUS:0030573972
SN - 0925-4439
VL - 1317
SP - 65
EP - 70
JO - Biochimica et Biophysica Acta - Molecular Basis of Disease
JF - Biochimica et Biophysica Acta - Molecular Basis of Disease
IS - 1
ER -