Uneven distribution of MHC class II epitopes within the influenza virus

Sherry R. Crowe, Shannon C. Miller, Deborah M. Brown, Pamela S. Adams, Richard W. Dutton, Allen G. Harmsen, Frances E. Lund, Troy D. Randall, Susan L. Swain, David L. Woodland

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

The identification of T cell epitopes is crucial for the understanding of the host immune response during infection. While much is known about the MHC class I-restricted response following influenza virus infection of C57BL/6 mice, with over 16 CD8 epitopes identified to date, less is known about the MHC class II-restricted response. Currently, only a few I-Ab-restricted T helper epitopes have been identified. Therefore, several important questions remain about how many class II epitopes exist in this system and whether these epitopes are evenly distributed within the most abundant viral proteins. In order to address these questions, we analyzed the repertoire of epitopes that drive the CD4+ T cell response to influenza virus infection in C57BL/6 (H-2b) mice. Using a panel of overlapping peptides from each of the viral proteins we show that approximately 20-30 epitopes drive the CD4 T cell response and that the majority of these peptides are derived from the NP and HA proteins. We were also able to demonstrate that vaccination with one of the newly identified epitopes, HA211-225/Ab, resulted in increased epitope-specific T cell numbers and a significant reduction in viral titers following influenza virus challenge.

Original languageEnglish (US)
Pages (from-to)457-467
Number of pages11
JournalVaccine
Volume24
Issue number4
DOIs
StatePublished - Jan 23 2006
Externally publishedYes

Keywords

  • Influenza
  • MHC II
  • T cells
  • Vaccination

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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  • Cite this

    Crowe, S. R., Miller, S. C., Brown, D. M., Adams, P. S., Dutton, R. W., Harmsen, A. G., Lund, F. E., Randall, T. D., Swain, S. L., & Woodland, D. L. (2006). Uneven distribution of MHC class II epitopes within the influenza virus. Vaccine, 24(4), 457-467. https://doi.org/10.1016/j.vaccine.2005.07.096