Upregulation of Npas4 protein expression by chronic administration of amphetamine in rat nucleus accumbens in vivo

Ming Lei Guo, Bing Xue, Dao Zhong Jin, Zhen Guo Liu, Eugene E. Fibuch, Li Min Mao, John Q. Wang

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The neuronal PAS domain protein 4 (Npas4) is a transcription factor that is almost exclusively expressed in the mammalian brain. As an activity-dependent transcription factor, Npas4 regulates the transcription of discrete genes and transcriptionally controls the experience-dependent learning and memory. In this study, we explored the impact of the psychostimulant amphetamine (AMPH) on Npas4 protein expression in the rat striatum. We found that acute systemic injection of AMPH had a minimal effect on protein levels of Npas4 in the caudate putamen (CPu) and nucleus accumbens (NAc), while AMPH readily increased protein products of the immediate early gene c-Fos in these regions. In contrast, repeated administration of AMPH (5. mg/kg, once daily for 5 days) triggered a significant increase in Npas4 expression in the NAc, although repeated AMPH did not alter Npas4 in the CPu. These data demonstrate that Npas4 is an AMPH-sensitive transcription factor. It is inducible selectively in the NAc in response to repeated AMPH administration.

Original languageEnglish (US)
Pages (from-to)210-214
Number of pages5
JournalNeuroscience Letters
Volume528
Issue number2
DOIs
StatePublished - Oct 24 2012

Keywords

  • C-Fos
  • Caudate
  • Immediate early gene
  • Le-PAS
  • NXF
  • PASD10
  • Striatum
  • Transcription factor

ASJC Scopus subject areas

  • Neuroscience(all)

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