Uptake and metabolism of biotin by human peripheral blood mononuclear cells

Janos Zempleni, Donald M. Mock

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

We studied the uptake of biotin into human peripheral blood mononuclear cells (PBMC) using [3H]biotin and studied the catabolism of biotin in PBMC using [14C]biotin. Over 30 min, [3H]biotin uptake was greater at 37°C than at 25°C (K(T) = 2.6 ± 0.4 nM, maximal velocity = 2.9 ± 0.2 fmol · 106 cells-1 · 30 min-1). Ouabain reduced [3H]biotin uptake to 65% of control values, suggesting that biotin uptake is Na-K-ATPase dependent. Unlabeled biotin and biotin analogs reduced the uptake of [3H]biotin to 22- 70% of control values, suggesting the presence of a competition for a structurally specific biotin transporter. When endocytosis by PBMC was stimulated by various acyl glycerols, [3H]biotin uptake was 40-73% of control values; these data are consistent with the hypothesis that stimulated endocytosis reduces biotin transporter density on the cell surface. During a 168-h incubation, PBMC did not catabolize [14C]biotin.

Original languageEnglish (US)
Pages (from-to)C382-C388
JournalAmerican Journal of Physiology - Cell Physiology
Volume275
Issue number2 44-2
DOIs
StatePublished - Aug 1998
Externally publishedYes

Keywords

  • 1,2-dioctanoyl-sn-glycerol
  • Endocytosis
  • Sodium-potassium- adenosinetriphosphatase

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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