@inbook{5dd71be1bbe543c4b9a161b933a39657,
title = "Use of a Ferret Model to Test Efficacy and Immunogenicity of Live Attenuated Mycobacterium avium Subspecies paratuberculosis Vaccines",
abstract = "Native hosts for the bacterial agent that causes Johne{\textquoteright}s disease are ruminants, which include cattle, sheep and goats among others. These large animals are often too costly to be used in testing experimental vaccines. In this chapter, we provide detailed methods to use an inexpensive and more manageable animal host, the ferret, to test efficacy and immunogenicity of live-attenuated Mycobacterium avium subspecies paratuberculosis (MAP) mutant strains prior to consideration as vaccine candidates.",
keywords = "Animal model, Ferrets, Johne{\textquoteright}s disease, Mycobacterium, Paratuberculosis",
author = "Bannantine, {John P.} and Tuhina Gupta and Zinniel, {Denise K.} and Ahmed Hikal and Quinn, {Frederick D.} and Barletta, {Raul G.}",
note = "Funding Information: This work was supported by the USDA Agricultural Research Service (JPB) and the USDA Hatch Multi State SAES University of Nebraska NEB 39-179 Project (RGB). Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1888-2_5",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "95--104",
booktitle = "Methods in Molecular Biology",
}