Use of a Ferret Model to Test Efficacy and Immunogenicity of Live Attenuated Mycobacterium avium Subspecies paratuberculosis Vaccines

John P. Bannantine, Tuhina Gupta, Denise K. Zinniel, Ahmed Hikal, Frederick D. Quinn, Raul G. Barletta

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Native hosts for the bacterial agent that causes Johne’s disease are ruminants, which include cattle, sheep and goats among others. These large animals are often too costly to be used in testing experimental vaccines. In this chapter, we provide detailed methods to use an inexpensive and more manageable animal host, the ferret, to test efficacy and immunogenicity of live-attenuated Mycobacterium avium subspecies paratuberculosis (MAP) mutant strains prior to consideration as vaccine candidates.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages95-104
Number of pages10
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2411
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Animal model
  • Ferrets
  • Johne’s disease
  • Mycobacterium
  • Paratuberculosis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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