Use of electroporation and conjugative mobilization for genetic manipulation of Staphylococcus epidermidis

Katherine L. Maliszewski, Austin S. Nuxoll

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

To perform mechanistic studies on the biology of bacteria including metabolism, physiology, and pathogenesis, it is essential to possess the tools required for genetic manipulation. Introduction of plasmid DNA into Staphylococcus epidermidis for subsequent genetic manipulation, including allelic replacement and complementation experiments, is typically performed by either electroporation or conjugative mobilization. Herein, standard protocols and tips for the transfer of plasmid DNA to S. epidermidis by these two methods are provided.

Original languageEnglish (US)
Title of host publicationStaphylococcus Epidermidis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages125-134
Number of pages10
ISBN (Print)9781627037358
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1106
ISSN (Print)1064-3745

Keywords

  • Competence
  • Conjugation
  • Conjugative mobilization
  • Electroporation
  • Staphylococcus
  • Staphylococcus epidermidis
  • Transformation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Maliszewski, K. L., & Nuxoll, A. S. (2014). Use of electroporation and conjugative mobilization for genetic manipulation of Staphylococcus epidermidis. In Staphylococcus Epidermidis: Methods and Protocols (pp. 125-134). (Methods in Molecular Biology; Vol. 1106). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-736-5_11