Use of NMR metabolomics to analyze the targets of D-cycloserine in mycobacteria: Role of D-alanine racemase

Steven Halouska; Ofelia Chacon; Robert J. Fenton; Denise K. Zinniel; Raul G. Barletta; Robert Powers

doi:10.1021/pr0704332

Use of NMR metabolomics to analyze the targets of D-cycloserine in mycobacteria: Role of D-alanine racemase

Steven Halouska, Ofelia Chacon, Robert J. Fenton, Denise K. Zinniel, Raul G. Barletta, Robert Powers

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

D-Cycloserine (DCS) is only used with multidrug-resistant strains of tuberculosis because of serious side effects. DCS is known to inhibit cell wall biosynthesis, but the in vivo lethal target is still unknown. We have applied NMR-based metabolomics combined with principal component analysis to monitor the in vivo effect of DCS on Mycobacterium smegmatis. Our analysis suggests DCS functions by inhibiting multiple protein targets.

Original language	English (US)
Pages (from-to)	4608-4614
Number of pages	7
Journal	Journal of proteome research
Volume	6
Issue number	12
DOIs	https://doi.org/10.1021/pr0704332
State	Published - Dec 2007

Keywords

D-alanine racemase
D-cycloserine
Metabolomics
Mycobacterium smegmatis
NMR
Principal component analysis
Tuberculosis

ASJC Scopus subject areas

Biochemistry
Chemistry(all)

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10.1021/pr0704332

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title = "Use of NMR metabolomics to analyze the targets of D-cycloserine in mycobacteria: Role of D-alanine racemase",

abstract = "D-Cycloserine (DCS) is only used with multidrug-resistant strains of tuberculosis because of serious side effects. DCS is known to inhibit cell wall biosynthesis, but the in vivo lethal target is still unknown. We have applied NMR-based metabolomics combined with principal component analysis to monitor the in vivo effect of DCS on Mycobacterium smegmatis. Our analysis suggests DCS functions by inhibiting multiple protein targets.",

keywords = "D-alanine racemase, D-cycloserine, Metabolomics, Mycobacterium smegmatis, NMR, Principal component analysis, Tuberculosis",

author = "Steven Halouska and Ofelia Chacon and Fenton, {Robert J.} and Zinniel, {Denise K.} and Barletta, {Raul G.} and Robert Powers",

year = "2007",

month = dec,

doi = "10.1021/pr0704332",

language = "English (US)",

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T1 - Use of NMR metabolomics to analyze the targets of D-cycloserine in mycobacteria

T2 - Role of D-alanine racemase

AU - Halouska, Steven

AU - Chacon, Ofelia

AU - Fenton, Robert J.

AU - Zinniel, Denise K.

AU - Barletta, Raul G.

AU - Powers, Robert

PY - 2007/12

Y1 - 2007/12

N2 - D-Cycloserine (DCS) is only used with multidrug-resistant strains of tuberculosis because of serious side effects. DCS is known to inhibit cell wall biosynthesis, but the in vivo lethal target is still unknown. We have applied NMR-based metabolomics combined with principal component analysis to monitor the in vivo effect of DCS on Mycobacterium smegmatis. Our analysis suggests DCS functions by inhibiting multiple protein targets.

AB - D-Cycloserine (DCS) is only used with multidrug-resistant strains of tuberculosis because of serious side effects. DCS is known to inhibit cell wall biosynthesis, but the in vivo lethal target is still unknown. We have applied NMR-based metabolomics combined with principal component analysis to monitor the in vivo effect of DCS on Mycobacterium smegmatis. Our analysis suggests DCS functions by inhibiting multiple protein targets.

KW - D-alanine racemase

KW - D-cycloserine

KW - Metabolomics

KW - Mycobacterium smegmatis

KW - NMR

KW - Principal component analysis

KW - Tuberculosis

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Use of NMR metabolomics to analyze the targets of D-cycloserine in mycobacteria: Role of D-alanine racemase

Abstract

Keywords

ASJC Scopus subject areas

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