Abstract
D-Cycloserine (DCS) is only used with multidrug-resistant strains of tuberculosis because of serious side effects. DCS is known to inhibit cell wall biosynthesis, but the in vivo lethal target is still unknown. We have applied NMR-based metabolomics combined with principal component analysis to monitor the in vivo effect of DCS on Mycobacterium smegmatis. Our analysis suggests DCS functions by inhibiting multiple protein targets.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4608-4614 |
| Number of pages | 7 |
| Journal | Journal of proteome research |
| Volume | 6 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1 2007 |
Keywords
- D-alanine racemase
- D-cycloserine
- Metabolomics
- Mycobacterium smegmatis
- NMR
- Principal component analysis
- Tuberculosis
ASJC Scopus subject areas
- Biochemistry
- Chemistry(all)
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Cite this
Halouska, S., Chacon, O., Fenton, R. J., Zinniel, D. K., Barletta, R. G., & Powers, R. (2007). Use of NMR metabolomics to analyze the targets of D-cycloserine in mycobacteria: Role of D-alanine racemase. Journal of proteome research, 6(12), 4608-4614. https://doi.org/10.1021/pr0704332