Use of S-adenosylmethionine as an index of methionine recycling in rat liver slices

Anthony J. Barak, Harriet C. Beckenhauer, Dean J. Tuma

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A simplified method for the measurement of methionine recycling in liver slices was developed. The method, a double-labeling procedure, utilizes S-adenosylmethionine as an index of recycling and offers several advantages over methionine, which has been used in other laboratories to determine methionine recycling in plants and intact animals. These advantages include (i) ease of isolation of index substance from tissue samples; (ii) elimination of oxidation problems prevalent with use of methionine; (iii) need for less initial concentrations of [methyl-3H] methionine and [35S]methionine used as tracers in the measurement of methionine recycling.

Original languageEnglish (US)
Pages (from-to)372-375
Number of pages4
JournalAnalytical Biochemistry
Volume127
Issue number2
DOIs
StatePublished - Dec 1982

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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