TY - JOUR
T1 - Use of S-adenosylmethionine as an index of methionine recycling in rat liver slices
AU - Barak, Anthony J.
AU - Beckenhauer, Harriet C.
AU - Tuma, Dean J.
PY - 1982/12
Y1 - 1982/12
N2 - A simplified method for the measurement of methionine recycling in liver slices was developed. The method, a double-labeling procedure, utilizes S-adenosylmethionine as an index of recycling and offers several advantages over methionine, which has been used in other laboratories to determine methionine recycling in plants and intact animals. These advantages include (i) ease of isolation of index substance from tissue samples; (ii) elimination of oxidation problems prevalent with use of methionine; (iii) need for less initial concentrations of [methyl-3H] methionine and [35S]methionine used as tracers in the measurement of methionine recycling.
AB - A simplified method for the measurement of methionine recycling in liver slices was developed. The method, a double-labeling procedure, utilizes S-adenosylmethionine as an index of recycling and offers several advantages over methionine, which has been used in other laboratories to determine methionine recycling in plants and intact animals. These advantages include (i) ease of isolation of index substance from tissue samples; (ii) elimination of oxidation problems prevalent with use of methionine; (iii) need for less initial concentrations of [methyl-3H] methionine and [35S]methionine used as tracers in the measurement of methionine recycling.
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U2 - 10.1016/0003-2697(82)90189-0
DO - 10.1016/0003-2697(82)90189-0
M3 - Article
C2 - 7165108
AN - SCOPUS:0020446329
SN - 0003-2697
VL - 127
SP - 372
EP - 375
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -