Erwinia chrysanthemi mutant CUCPB5047, Δ(pelA pelE) Δ(pelB pelC)::28bp Δ(pelX) Δ4bp pehX::ΩCm(r), was constructed, mutated with Tn5tac1, and screened for isopropyl-β-D-thiogalactopyranoside-dependent pectate lyase (Pel) production. AKm(r) SacI fragment from the hyperexpressing Pel+ mutant CUCPB5066 was cloned into Escherichia coli and sequenced. The gene identified, pelL, encodes a novel, asparagine-rich, highly alkaline enzyme that is similar in primary structure to PelX and in enzymological properties to PelE.
ASJC Scopus subject areas
- Molecular Biology