@article{8a268bc3f0c441269e3e12cdd400a291,
title = "Using EuGeneCiD and EuGeneCiM computational tools for synthetic biology",
abstract = "Synthetic biology often relies on the design of genetic circuits, utilizing “bioparts” (modular DNA pieces) to accomplish desired responses to external stimuli. While such designs are usually intuited, detailed here is a computational approach to synthetic biology design and modeling using optimization-based tools named Eukaryotic Genetic Circuit Design and Modeling. These allow for designing and subsequent screening of genetic circuits to increase the chances of in vivo success and contribute to the development of an application development pipeline. For complete details on the use and execution of this protocol, please refer to Schroeder, Baber, and Saha (2021).",
keywords = "Biotechnology and bioengineering, Systems biology",
author = "Schroeder, {Wheaton L.} and Baber, {Anna S.} and Rajib Saha",
note = "Funding Information: This work has been completed utilizing the Holland Computing Center of the University of Nebraska, which receives support from the Nebraska Research Initiative . The authors gratefully acknowledge funding from the NSF EPSCoR Center for Root and Rhizobiome Innovation Grant 25-1215-0139-025 at the University of Nebraska – Lincoln. The authors would also like to acknowledge the contribution of participants of the Young Nebraska Scientists Program (YNSP) who participated in this research including (listing in alphabetical order by family name): Bree Brunsman, Evan Fulton, Ali Keshk, Kareem Keshk, and Molly Nora. Funding Information: This work has been completed utilizing the Holland Computing Center of the University of Nebraska, which receives support from the Nebraska Research Initiative. The authors gratefully acknowledge funding from the NSF EPSCoR Center for Root and Rhizobiome Innovation Grant 25-1215-0139-025 at the University of Nebraska ? Lincoln. The authors would also like to acknowledge the contribution of participants of the Young Nebraska Scientists Program (YNSP) who participated in this research including (listing in alphabetical order by family name): Bree Brunsman, Evan Fulton, Ali Keshk, Kareem Keshk, and Molly Nora. Conceptualization, W.L.S. and R.S.; data curation, investigation, formal analysis, and software, W.L.S. and A.S.B.; funding acquisition, R.S.; methodology, W.L.S.; project administration, resources, and supervision, R.S.; validation and visualization, W.L.S.; writing ? original draft, W.L.S. and R.S.; writing ? reviewing & editing ? W.L.S. A.S.B. and R.S. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = dec,
day = "17",
doi = "10.1016/j.xpro.2021.100820",
language = "English (US)",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "4",
}