Using the incomplete genome of the ectomycorrhizal fungus Amanita bisporigera to identify molecular polymorphisms in the related Amanita phalloides

Rachel I. Adams, Heather E. Hallen, Anne Pringle

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We describe the cross-genomic isolation of 13 single nucleotide polymorphisms (SNPs) and one variable microsatellite from five loci for the death cap mushroom Amanita phalloides. Microsatellite repeats were identified by searching the partial Amanita bisporigera genome. Flanking primers were designed for 25 of these microsatellite loci and tested for cross-amplification in A. phalloides. One locus contained an interrupted, compound microsatellite, and four loci contained one to six SNPs. These results demonstrate the usefulness of even an incomplete genome to identify molecular markers for population studies in nonmodel organisms.

Original languageEnglish (US)
Pages (from-to)218-220
Number of pages3
JournalMolecular Ecology Notes
Volume6
Issue number1
DOIs
StatePublished - Mar 2006

Keywords

  • Amanita phalloides
  • Ectomycorrhizal fungus
  • Fungal genome
  • Single nucleotide polymorphism

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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