@inbook{1059d81a7ced4ea6a8102a9354138370,
title = "Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cells",
abstract = "Protein misfolding and aggregation is now recognized as a hallmark of numerous human diseases. Standard bioanalytical techniques for monitoring protein aggregation generally rely on small molecules that provide an optical readout of fibril formation. While these methods have been useful for mechanistic studies, additional approaches are required to probe the equilibrium between soluble and insoluble protein within living systems. Such approaches could provide platforms for the identification of inhibitors of protein aggregation as well as a means to investigate the effect of mutations on protein aggregation in model systems. In this chapter, we provide detailed protocols for employing split-NanoLuc luciferase (Nluc) fragments to monitor changes in protein solubility in bacterial and mammalian cells. This sensitive luminesce-based assay can report upon changes in protein solubility induced by inhibitors and disease-relevant mutations.",
keywords = "Aggregation inhibitor, Luciferase, Luminescence, Protein solubility, Split-protein reassembly",
author = "Nelson, {Travis J.} and Jia Zhao and Stains, {Cliff I.}",
note = "Funding Information: This work was supported by the NIH (R35GM119751). The content of this work is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
doi = "10.1016/bs.mie.2019.02.003",
language = "English (US)",
isbn = "9780128181195",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "55--66",
editor = "Shukla, {Arun K.}",
booktitle = "Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part B",
}