UV-triggered affinity capture identifies interactions between the Plasmodium falciparum multidrug resistance protein 1 (PfMDR1) and antimalarial agents in live parasitized cells

Ralf Brunner, Caroline L. Ng, Hamed Aissaoui, Myles H. Akabas, Christoph Boss, Reto Brun, Paul S. Callaghan, Olivier Corminboeuf, David A. Fidock, Ithiel J. Frame, Bibia Heidmann, Amélie Le Bihan, Paul Jenö, Corinna Mattheis, Suzette Moes, Ingrid B. Müller, Michelle Paguio, Paul D. Roepe, Romain Siegrist, Till VossRichard W.D. Welford, Sergio Wittlin, Christoph Binkert

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Background: We have previously identified potent novel antimalarial compounds with an unknown mode of action. Results: A photo-reactive affinity capture method was used to identify parasite proteins that interact with these antimalarials. Conclusion: ACT-213615 interacts with Plasmodium falciparum multidrug resistance protein 1 (PfMDR1). Significance: This photo-reactive affinity capture method can be generally used to identify drug targets in live cells.

Original languageEnglish (US)
Pages (from-to)22576-22583
Number of pages8
JournalJournal of Biological Chemistry
Volume288
Issue number31
DOIs
StatePublished - Aug 2 2013
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Brunner, R., Ng, C. L., Aissaoui, H., Akabas, M. H., Boss, C., Brun, R., Callaghan, P. S., Corminboeuf, O., Fidock, D. A., Frame, I. J., Heidmann, B., Bihan, A. L., Jenö, P., Mattheis, C., Moes, S., Müller, I. B., Paguio, M., Roepe, P. D., Siegrist, R., ... Binkert, C. (2013). UV-triggered affinity capture identifies interactions between the Plasmodium falciparum multidrug resistance protein 1 (PfMDR1) and antimalarial agents in live parasitized cells. Journal of Biological Chemistry, 288(31), 22576-22583. https://doi.org/10.1074/jbc.M113.453159