Abstract
Experimental design principles for achieving validity and efficiency are required for any multi-variable experiment. In this chapter we attempt to review validation principles applicable to complex high-throughput experiments such as proteomic profiling. Typical proteomic study consists of a string of multiple steps each of which can be validated by different orthogonal methods. Nevertheless, all steps will have an impact on the overall outcome, providing answers of biological importance. As much as validation of analytical methods is quite well established, validation of experimental design or bioinformatics analysis is still in its infancy. We also discuss the validation of proteomic profiling experiments from the perspective of regulatory affairs.
Original language | English (US) |
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Title of host publication | Proteomic Profiling and Analytical Chemistry |
Subtitle of host publication | The Crossroads: Second Edition |
Publisher | Elsevier Inc. |
Pages | 253-271 |
Number of pages | 19 |
ISBN (Print) | 9780444636881 |
DOIs | |
State | Published - Mar 22 2016 |
Keywords
- Bioinformatics
- Cross-validation
- Detection level
- Instrument validation
- Precision
- Regulation
- Reproducibility
- Sample loss
- Validation, Accuracy
ASJC Scopus subject areas
- Chemistry(all)