Vancomycin serum protein binding determination by ultrafiltration

B. H. Ackerman, E. H. Taylor, K. M. Olsen, W. Abdel-Malak, A. A. Pappas

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Sixty-two serum concentrations were obtained from 12 infected patients enrolled in a vancomycin pharmacokinetic study. Both unbound and total serum vancomycin concentrations were measured using ultrafiltration and a commercial fluorescent polarization immunoassay. Ultrafiltrates were obtained by centrifugation at 1000 x g for ten minutes at room temperature and their assay indicated a range in protein binding from 7.9 to 71 percent. The mean protein binding (mean ± SD) was 41.95 ± 14.15 percent. No measurable adsorption of vancomycin onto the ultrafiltration membrane was noted. Orthogonal regression of unbound versus total vancomycin concentrations was described by the equation y = 0.597x-0.362 with a correlation coefficient of 0.948.

Original languageEnglish (US)
Pages (from-to)300-303
Number of pages4
JournalDrug Intelligence and Clinical Pharmacy
Volume22
Issue number4
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

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