TY - JOUR
T1 - Varying efficiency of long-term replication of papillomaviruses in Saccharomyces cerevisiae
AU - Rogers, Adam J.
AU - Loggen, Malte
AU - Lee, Karen
AU - Angeletti, Peter C.
N1 - Funding Information:
We thank the Angeletti lab members for valuable discussion and critique of this work. This work was supported by NIH Grant: K01CA100736 to P.C.A. and by a COBRE grant (5P20RR015635) through the Nebraska Center for Virology.
PY - 2008/11/10
Y1 - 2008/11/10
N2 - Human papillomaviruses (HPVs) replicate in mitotically active basal keratinocytes. Two virally encoded proteins, E1, a helicase, and E2, a transcription factor, are important players in replication and maintenance of HPV episomes. We previously showed that HPV16 could replicate stably in Saccharomyces cerevisiae [Angeletti, P.C., Kim, K., Fernandes, F.J., and Lambert, P.F. (2002)] and we identified cis-elements that mediate replication and maintenance [J. Virol. 76(7), 3350-3358.; Kim, K., Angeletti, P.C., Hassebroek, E.C., and Lambert, P.F. (2005)]. Here, we demonstrate that although multiple HPV genomes replicate stably in yeast, they do so with differing long-term efficiency; HPV6-Ura3 is replicated at the highest copy number, followed by HPV31-Ura3 and HPV16-Ura3 respectively, HPV11-Ura3 and HPV18-Ura3 were unable replicate without the presence of E2 expression and BPV-1-Ura3 was unable to replicate, with or without the presence of E2. These studies suggest genotype-specific differences in HPV replication and maintenance.
AB - Human papillomaviruses (HPVs) replicate in mitotically active basal keratinocytes. Two virally encoded proteins, E1, a helicase, and E2, a transcription factor, are important players in replication and maintenance of HPV episomes. We previously showed that HPV16 could replicate stably in Saccharomyces cerevisiae [Angeletti, P.C., Kim, K., Fernandes, F.J., and Lambert, P.F. (2002)] and we identified cis-elements that mediate replication and maintenance [J. Virol. 76(7), 3350-3358.; Kim, K., Angeletti, P.C., Hassebroek, E.C., and Lambert, P.F. (2005)]. Here, we demonstrate that although multiple HPV genomes replicate stably in yeast, they do so with differing long-term efficiency; HPV6-Ura3 is replicated at the highest copy number, followed by HPV31-Ura3 and HPV16-Ura3 respectively, HPV11-Ura3 and HPV18-Ura3 were unable replicate without the presence of E2 expression and BPV-1-Ura3 was unable to replicate, with or without the presence of E2. These studies suggest genotype-specific differences in HPV replication and maintenance.
KW - Extrachromosal DNA
KW - Human papillomavirus
KW - Persistent infection
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U2 - 10.1016/j.virol.2008.08.038
DO - 10.1016/j.virol.2008.08.038
M3 - Article
C2 - 18829061
AN - SCOPUS:54049148489
VL - 381
SP - 6
EP - 10
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -