Vascular endothelial growth factor (rhVEGF165) stimulates direct angiogenesis in the rabbit cornea

G. D. Phillips, A. M. Stone, B. D. Jones, J. C. Schultz, R. A. Whitehead, D. R. Knighton

Research output: Contribution to journalArticlepeer-review

121 Scopus citations


The object of this study was to test vascular endothelial- growth factor (VEGF) for angiogenic activity in the rabbit corneal assay. VEGF doses ranging from 20ng to 1000ng were incorporated into a slow release polymer and implanted into the avascular rabbit cornea. Capillary formation in the cornea was visually analyzed on a daily basis and examined with histology, transmission electron microscopy and scanning electron microscopy of vascular corrosion casts on days 2 and 7 post-implantation. VEGF implants (200ng to 1000ng) consistently stimulated angiogenesis. This neovascularization occurred in the absence of inflammation. We conclude that VEGF acts directly on endothelial cells, initiating and mediating the formation of capillaries.

Original languageEnglish (US)
Pages (from-to)961-966
Number of pages6
JournalIn Vivo
Issue number6
StatePublished - 1994

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • Pharmacology


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