TY - JOUR
T1 - Very-long-chain fatty acids are involved in polar auxin transport and developmental patterning in Arabidopsis
AU - Roudier, François
AU - Gissot, Lionel
AU - Beaudoin, Frédéric
AU - Haslam, Richard
AU - Michaelson, Louise
AU - Marion, Jessica
AU - Molino, Diana
AU - Lima, Amparo
AU - Bach, Liên
AU - Morin, Halima
AU - Tellier, Frédérique
AU - Palauqui, Jean Christophe
AU - Bellec, Yannick
AU - Renne, Charlotte
AU - Miquel, Martine
AU - DaCosta, Marco
AU - Vignard, Julien
AU - Rochat, Christine
AU - Markham, Jonathan E.
AU - Moreau, Patrick
AU - Napier, Johnathan
AU - Faure, Jean Denis
PY - 2010/2
Y1 - 2010/2
N2 - Very-long-chain fatty acids (VLCFAs) are essential for many aspects of plant development and necessary for the synthesis of seed storage triacylglycerols, epicuticular waxes, and sphingolipids. Identification of the acetyl-CoA carboxylase PASTICCINO3 and the 3-hydroxy acyl-CoA dehydratase PASTICCINO2 revealed that VLCFAs are important for cell proliferation and tissue patterning. Here, we show that the immunophilin PASTICCINO1 (PAS1) is also required for VLCFA synthesis. Impairment of PAS1 function results in reduction of VLCFA levels that particularly affects the composition of sphingolipids, known to be important for cell polarity in animals. Moreover, PAS1 associates with several enzymes of the VLCFA elongase complex in the endoplasmic reticulum. The pas1 mutants are deficient in lateral root formation and are characterized by an abnormal patterning of the embryo apex, which leads to defective cotyledon organogenesis. Our data indicate that in both tissues, defective organogenesis is associated with the mistargeting of the auxin efflux carrier PIN FORMED1 in specific cells, resulting in local alteration of polar auxin distribution. Furthermore, we show that exogenous VLCFAs rescue lateral root organogenesis and polar auxin distribution, indicating their direct involvement in these processes. Based on these data, we propose that PAS1 acts as a molecular scaffold for the fatty acid elongase complex in the endoplasmic reticulum and that the resulting VLCFAs are required for polar auxin transport and tissue patterning during plant development.
AB - Very-long-chain fatty acids (VLCFAs) are essential for many aspects of plant development and necessary for the synthesis of seed storage triacylglycerols, epicuticular waxes, and sphingolipids. Identification of the acetyl-CoA carboxylase PASTICCINO3 and the 3-hydroxy acyl-CoA dehydratase PASTICCINO2 revealed that VLCFAs are important for cell proliferation and tissue patterning. Here, we show that the immunophilin PASTICCINO1 (PAS1) is also required for VLCFA synthesis. Impairment of PAS1 function results in reduction of VLCFA levels that particularly affects the composition of sphingolipids, known to be important for cell polarity in animals. Moreover, PAS1 associates with several enzymes of the VLCFA elongase complex in the endoplasmic reticulum. The pas1 mutants are deficient in lateral root formation and are characterized by an abnormal patterning of the embryo apex, which leads to defective cotyledon organogenesis. Our data indicate that in both tissues, defective organogenesis is associated with the mistargeting of the auxin efflux carrier PIN FORMED1 in specific cells, resulting in local alteration of polar auxin distribution. Furthermore, we show that exogenous VLCFAs rescue lateral root organogenesis and polar auxin distribution, indicating their direct involvement in these processes. Based on these data, we propose that PAS1 acts as a molecular scaffold for the fatty acid elongase complex in the endoplasmic reticulum and that the resulting VLCFAs are required for polar auxin transport and tissue patterning during plant development.
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U2 - 10.1105/tpc.109.071209
DO - 10.1105/tpc.109.071209
M3 - Article
C2 - 20145257
AN - SCOPUS:77950365776
SN - 1040-4651
VL - 22
SP - 364
EP - 375
JO - Plant Cell
JF - Plant Cell
IS - 2
ER -