Virulence markers in the 5' untranslated region of genotype 2 bovine viral diarrhea virus isolates

Christina L. Topliff, Clayton L. Kelling

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Virulence markers to distinguish high from low virulence bovine viral diarrhea virus genotype 2 isolates have not been previously reported. The objective of this study was to identify virulence markers by evaluating the primary and secondary structures of the 5'-untranslated region of low and high virulence bovine viral diarrhea virus genotype 2 isolates. The nucleotide sequences of the entire 5'-untranslated region mRNA of eight bovine viral diarrhea virus genotype 2 isolates, four of high virulence and four of low virulence, and two genotype 1 reference isolates were determined using a polymerase chain reaction and a 5' Rapid Amplification of cDNA Ends System. Two nucleotide substitutions were identified in the internal ribosomal entry site that distinguished the high virulence from the low virulence genotype 2 isolates. The low virulence isolates had a cytosine at position 219, whereas the high virulence isolates had a uracil. At position 278, a uracil or cytosine was found in the low and high virulence groups, respectively. The substituted bases are virulence markers that were used to identify bovine viral diarrhea virus genotype 2 isolates of high virulence.

Original languageEnglish (US)
Pages (from-to)164-172
Number of pages9
JournalVirology
Volume250
Issue number1
DOIs
StatePublished - Oct 10 1998

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Virulence markers in the 5' untranslated region of genotype 2 bovine viral diarrhea virus isolates'. Together they form a unique fingerprint.

  • Cite this