Virus detection by nucleic acid hybridization: Examination of normal and ALS tissues for the presence of poliovirus

D. E. Kohne; C. J. Gibbs; L. White; S. M. Tracy; W. Meinke; R. A. Smith

doi:10.1099/0022-1317-56-2-223

Virus detection by nucleic acid hybridization: Examination of normal and ALS tissues for the presence of poliovirus

D. E. Kohne, C. J. Gibbs, L. White, S. M. Tracy, W. Meinke, R. A. Smith

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34 Scopus citations

Abstract

A nucleic acid hybridization assay was developed as a sensitive assay for the presence of poliovirus RNA in human tissue. The assay could detect the presence of an average of one poliovirus per 200 cells. A method for determining the extent of degradtion of the tissue RNA was developed and used to show that a significant fraction of human central nervous system (CNS) autopsy material contains highly degraded RNA which is unsuitable for hybridization studies. A total of 15 different control and amyotrophic lateral sclerosis tissues were assayed for the presence of poliovirus-like RNA. Virus RNA was detected in one of the control tissues and in none of the ALS tissues.

Original language	English (US)
Pages (from-to)	223-233
Number of pages	11
Journal	Unknown Journal
Volume	56
Issue number	2
DOIs	https://doi.org/10.1099/0022-1317-56-2-223
State	Published - 1981
Externally published	Yes

ASJC Scopus subject areas

Virology

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abstract = "A nucleic acid hybridization assay was developed as a sensitive assay for the presence of poliovirus RNA in human tissue. The assay could detect the presence of an average of one poliovirus per 200 cells. A method for determining the extent of degradtion of the tissue RNA was developed and used to show that a significant fraction of human central nervous system (CNS) autopsy material contains highly degraded RNA which is unsuitable for hybridization studies. A total of 15 different control and amyotrophic lateral sclerosis tissues were assayed for the presence of poliovirus-like RNA. Virus RNA was detected in one of the control tissues and in none of the ALS tissues.",

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AU - Kohne, D. E.

AU - Gibbs, C. J.

AU - White, L.

AU - Tracy, S. M.

AU - Meinke, W.

AU - Smith, R. A.

PY - 1981

Y1 - 1981

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AB - A nucleic acid hybridization assay was developed as a sensitive assay for the presence of poliovirus RNA in human tissue. The assay could detect the presence of an average of one poliovirus per 200 cells. A method for determining the extent of degradtion of the tissue RNA was developed and used to show that a significant fraction of human central nervous system (CNS) autopsy material contains highly degraded RNA which is unsuitable for hybridization studies. A total of 15 different control and amyotrophic lateral sclerosis tissues were assayed for the presence of poliovirus-like RNA. Virus RNA was detected in one of the control tissues and in none of the ALS tissues.

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Virus detection by nucleic acid hybridization: Examination of normal and ALS tissues for the presence of poliovirus

Abstract

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