Virus detection by nucleic acid hybridization: Examination of normal and ALS tissues for the presence of poliovirus

D. E. Kohne, C. J. Gibbs, L. White, S. M. Tracy, W. Meinke, R. A. Smith

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

A nucleic acid hybridization assay was developed as a sensitive assay for the presence of poliovirus RNA in human tissue. The assay could detect the presence of an average of one poliovirus per 200 cells. A method for determining the extent of degradtion of the tissue RNA was developed and used to show that a significant fraction of human central nervous system (CNS) autopsy material contains highly degraded RNA which is unsuitable for hybridization studies. A total of 15 different control and amyotrophic lateral sclerosis tissues were assayed for the presence of poliovirus-like RNA. Virus RNA was detected in one of the control tissues and in none of the ALS tissues.

Original languageEnglish (US)
Pages (from-to)223-233
Number of pages11
JournalUnknown Journal
Volume56
Issue number2
DOIs
StatePublished - 1981

ASJC Scopus subject areas

  • Virology

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