Virus infection of culturable Chlorella-like algae and development of a plaque assay

James L. Van Etten; Dwight E. Burbank; Daniel Kuczmarski; Russel H. Meints

doi:10.1126/science.219.4587.994

Virus infection of culturable Chlorella-like algae and development of a plaque assay

James L. Van Etten, Dwight E. Burbank, Daniel Kuczmarski, Russel H. Meints

Plant Pathology

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Abstract

Four distinct viruses with double-stranded DNA are known to replicate in Chlorella-like algae symbiotic with hydras and paramecia. An attempt was made to infect a number of cultured Chlorella strains derived from invertebrate hosts with these viruses. One of the viruses, PBCV-1, replicated in two of the algal strains. Restriction endonuclease analysis of the viral DNA showed that the infectious progeny virus was identical to the input virus; thus, Koch's postulates were fulfilled. Viral infection of the two Chlorella strains has allowed the large-scale production of a eukaryotic algal virus and the development of a plaque assay for the virus.

Original language	English (US)
Pages (from-to)	994-996
Number of pages	3
Journal	Science
Volume	219
Issue number	4587
DOIs	https://doi.org/10.1126/science.219.4587.994
State	Published - 1983

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abstract = "Four distinct viruses with double-stranded DNA are known to replicate in Chlorella-like algae symbiotic with hydras and paramecia. An attempt was made to infect a number of cultured Chlorella strains derived from invertebrate hosts with these viruses. One of the viruses, PBCV-1, replicated in two of the algal strains. Restriction endonuclease analysis of the viral DNA showed that the infectious progeny virus was identical to the input virus; thus, Koch's postulates were fulfilled. Viral infection of the two Chlorella strains has allowed the large-scale production of a eukaryotic algal virus and the development of a plaque assay for the virus.",

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AU - Kuczmarski, Daniel

AU - Meints, Russel H.

PY - 1983

Y1 - 1983

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AB - Four distinct viruses with double-stranded DNA are known to replicate in Chlorella-like algae symbiotic with hydras and paramecia. An attempt was made to infect a number of cultured Chlorella strains derived from invertebrate hosts with these viruses. One of the viruses, PBCV-1, replicated in two of the algal strains. Restriction endonuclease analysis of the viral DNA showed that the infectious progeny virus was identical to the input virus; thus, Koch's postulates were fulfilled. Viral infection of the two Chlorella strains has allowed the large-scale production of a eukaryotic algal virus and the development of a plaque assay for the virus.

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Virus infection of culturable Chlorella-like algae and development of a plaque assay

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