TY - JOUR
T1 - Visualizing small differences using subtractive chromatographic analysis
AU - Zawatzky, Kerstin
AU - Reibarkh, Mikhail
AU - Canfield, Nicole
AU - Wang, Ting Chuan
AU - Li, Shanren
AU - Du, Liangcheng
AU - Welch, Christopher J.
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/10/14
Y1 - 2016/10/14
N2 - Subtraction of chromatograms coming from two different samples collected under identical conditions can highlight small variations, serving as a useful tool for visualizing differences between experimental and control groups. While the basis for this general approach has been known for decades, the technique is seldom used in modern chromatographic analysis. We report an investigation into the application of subtractive chromatographic analysis in several areas of pharmaceutical research where detection of small differences between samples is important. Our investigation found that elimination of artifacts caused by peak misalignment was often necessary, especially for extremely sharp chromatographic peaks obtained in rapid injection MISER chromatography. Alignment of individual peaks prior to subtraction, combined with fast detector sampling rates, or data interpolation in cases where this is not possible, was found to afford convenient visualization of small differences (∼1%) among samples, suggesting potential utility in high throughput screening of process adsorbents or other applications in pharmaceutical research and development.
AB - Subtraction of chromatograms coming from two different samples collected under identical conditions can highlight small variations, serving as a useful tool for visualizing differences between experimental and control groups. While the basis for this general approach has been known for decades, the technique is seldom used in modern chromatographic analysis. We report an investigation into the application of subtractive chromatographic analysis in several areas of pharmaceutical research where detection of small differences between samples is important. Our investigation found that elimination of artifacts caused by peak misalignment was often necessary, especially for extremely sharp chromatographic peaks obtained in rapid injection MISER chromatography. Alignment of individual peaks prior to subtraction, combined with fast detector sampling rates, or data interpolation in cases where this is not possible, was found to afford convenient visualization of small differences (∼1%) among samples, suggesting potential utility in high throughput screening of process adsorbents or other applications in pharmaceutical research and development.
KW - High throughput analysis
KW - Multiple injections in a single experimental run
KW - Peak alignment
KW - Subtractive chromatography
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U2 - 10.1016/j.chroma.2016.09.025
DO - 10.1016/j.chroma.2016.09.025
M3 - Article
C2 - 27663729
AN - SCOPUS:84994476610
SN - 0021-9673
VL - 1468
SP - 245
EP - 249
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -