Abstract
Exposure to organic dusts elicits airway inflammatory diseases. Vitamin D recently has been associated with various airway inflammatory diseases, but its role in agricultural organic dust exposures is unknown. This study investigated whether vitamin D reduces organic dust-induced inflammatory outcomes in cell culture and animal models. Organic dust extracts obtained from swine confinement facilities induced neutrophil chemokine production (human IL-8, murine CXCL1/CXCL2). Neutrophil chemokine induction was reduced in human blood monocytes, human bronchial epithelial cells, and murine lung slices pretreated with 1,25-(OH)2D3. Intranasal inhalation of organic dust extract induced neutrophil influx, and CXCL1/CXCL2 release was also decreased in mice fed a relatively high vitamin D diet as compared to mice fed a low vitamin D diet. These findings were associated with reduced tracheal epithelial cell PKCα and PKCε activity and whole lung TLR2 and TLR4 gene expression. Collectively, vitamin D plays a role in modulating organic dust-induced airway inflammatory outcomes.
Original language | English (US) |
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Pages (from-to) | 77-86 |
Number of pages | 10 |
Journal | Journal of Biochemical and Molecular Toxicology |
Volume | 27 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2013 |
Keywords
- Epithelial
- Farm
- Lung
- Neutrophil
- Organic dust
- TLR
- Vitamin D
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Toxicology
- Health, Toxicology and Mutagenesis