Vitamin D Treatment Modulates Organic Dust-Induced Cellular and Airway Inflammatory Consequences

Gregory A. Golden, Todd A. Wyatt, Debra J. Romberger, Daniel Reiff, Michael McCaskill, Christopher Bauer, Angela M. Gleason, Jill A. Poole

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Exposure to organic dusts elicits airway inflammatory diseases. Vitamin D recently has been associated with various airway inflammatory diseases, but its role in agricultural organic dust exposures is unknown. This study investigated whether vitamin D reduces organic dust-induced inflammatory outcomes in cell culture and animal models. Organic dust extracts obtained from swine confinement facilities induced neutrophil chemokine production (human IL-8, murine CXCL1/CXCL2). Neutrophil chemokine induction was reduced in human blood monocytes, human bronchial epithelial cells, and murine lung slices pretreated with 1,25-(OH)2D3. Intranasal inhalation of organic dust extract induced neutrophil influx, and CXCL1/CXCL2 release was also decreased in mice fed a relatively high vitamin D diet as compared to mice fed a low vitamin D diet. These findings were associated with reduced tracheal epithelial cell PKCα and PKCε activity and whole lung TLR2 and TLR4 gene expression. Collectively, vitamin D plays a role in modulating organic dust-induced airway inflammatory outcomes.

Original languageEnglish (US)
Pages (from-to)77-86
Number of pages10
JournalJournal of Biochemical and Molecular Toxicology
Volume27
Issue number1
DOIs
StatePublished - Jan 2013

Keywords

  • Epithelial
  • Farm
  • Lung
  • Neutrophil
  • Organic dust
  • TLR
  • Vitamin D

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Toxicology
  • Health, Toxicology and Mutagenesis

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