TY - JOUR
T1 - Vitamin deficits in severe alcoholic fatty liver of man calculated from multiple reference units
AU - Frank, Oscar
AU - Luisada-Opper, Anita
AU - Sorrell, Michael F.
AU - Thomson, Allan D.
AU - Baker, Herman
PY - 1971/10
Y1 - 1971/10
N2 - Liver tissue levels of thiamine, pantothenate, biotin, vitamin B12, total folates, nicotinate, and vitamin B6 were determined in biopsy and autopsy specimens from 69 patients with normal liver and 44 patients with severe fatty liver. These results define normal liver tissue B-complex vitamin levels in man and demonstrate a significant reduction of all the vitamins when the liver is severely infiltrated with fat. When vitamin levels in liver were related to dry weight, total fats, total nitrogen, and DNA-phosphorus as reference bases, vitamin titers in order of decreasing magnitude in normal livers were nicotinate, pantothenate, B6, thiamine, N5-methyltetrahydrofolates, other tetrahydrofolates, B12, and biotin. In the severe fatty liver pantothenate was higher than nicotinate and other tetrahydrofolates were higher than N5-methyltetrahydrofolates. Biotin was markedly decreased in the severe fatty liver when dry weight, total fat, total nitrogen, and DNA-phosphorus were each used as reference base; however, the thiamine and N5-methyltetrahydrofolate pattern varied if dry weight alone was used as reference base. Total liver fat compared favorably with total nitrogen and DNA-phosphorus as a reference base. It showed close agreement with, on one hand, tissue vitamin decreases and on the other hand, with histologically demonstrable fat.
AB - Liver tissue levels of thiamine, pantothenate, biotin, vitamin B12, total folates, nicotinate, and vitamin B6 were determined in biopsy and autopsy specimens from 69 patients with normal liver and 44 patients with severe fatty liver. These results define normal liver tissue B-complex vitamin levels in man and demonstrate a significant reduction of all the vitamins when the liver is severely infiltrated with fat. When vitamin levels in liver were related to dry weight, total fats, total nitrogen, and DNA-phosphorus as reference bases, vitamin titers in order of decreasing magnitude in normal livers were nicotinate, pantothenate, B6, thiamine, N5-methyltetrahydrofolates, other tetrahydrofolates, B12, and biotin. In the severe fatty liver pantothenate was higher than nicotinate and other tetrahydrofolates were higher than N5-methyltetrahydrofolates. Biotin was markedly decreased in the severe fatty liver when dry weight, total fat, total nitrogen, and DNA-phosphorus were each used as reference base; however, the thiamine and N5-methyltetrahydrofolate pattern varied if dry weight alone was used as reference base. Total liver fat compared favorably with total nitrogen and DNA-phosphorus as a reference base. It showed close agreement with, on one hand, tissue vitamin decreases and on the other hand, with histologically demonstrable fat.
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U2 - 10.1016/0014-4800(71)90098-0
DO - 10.1016/0014-4800(71)90098-0
M3 - Article
C2 - 5111801
AN - SCOPUS:0015143732
VL - 15
SP - 191
EP - 197
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
SN - 0014-4800
IS - 2
ER -