Volatile carbonyl levels in tissues of transgenic mice with nerve sheath tumors

Susan E. Ebeler; Steven H. Hinrichs; Andrew J. Clifford; Takayuki Shibamoto

doi:10.1016/0378-4347(93)E0436-T

Volatile carbonyl levels in tissues of transgenic mice with nerve sheath tumors

Susan E. Ebeler, Steven H. Hinrichs, Andrew J. Clifford, Takayuki Shibamoto

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Volatile carbonyl compounds in homogenates prepared from various tissues of tumor-bearing transgenic mice were determined. Formaldehyde and acetaldehyde were derivatized to thiazolidines. Malonaldehyde was derivatized to 1-methylpyrazole. The derivatives were quantified by gas chromatography with a highly sensitive and specific nitrogen-phosphorus detector. The limits of quantitation of formaldehyde and malonaldehyde were 2 μg/ml of homogenate and 27 ng/ml of homogenate, respectively. Levels of malonaldehyde in the erythrocytes and gastrocnemius of tumor-bearing transgenic mice were elevated as compared to the same tissue in control non-transgenic mice. Brain, liver, kidney, heart, and spleen tissues of the tumor-bearing mice exhibited decreased malonaldehyde levels. Similar results were obtained for formaldehyde and acetaldehyde.

Original language	English (US)
Pages (from-to)	9-18
Number of pages	10
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	654
Issue number	1
DOIs	https://doi.org/10.1016/0378-4347(93)E0436-T
State	Published - Mar 18 1994

ASJC Scopus subject areas

General Chemistry

Access to Document

10.1016/0378-4347(93)E0436-T

Cite this

@article{4cb0708e431c42b6beb17151da5e1e9d,

title = "Volatile carbonyl levels in tissues of transgenic mice with nerve sheath tumors",

abstract = "Volatile carbonyl compounds in homogenates prepared from various tissues of tumor-bearing transgenic mice were determined. Formaldehyde and acetaldehyde were derivatized to thiazolidines. Malonaldehyde was derivatized to 1-methylpyrazole. The derivatives were quantified by gas chromatography with a highly sensitive and specific nitrogen-phosphorus detector. The limits of quantitation of formaldehyde and malonaldehyde were 2 μg/ml of homogenate and 27 ng/ml of homogenate, respectively. Levels of malonaldehyde in the erythrocytes and gastrocnemius of tumor-bearing transgenic mice were elevated as compared to the same tissue in control non-transgenic mice. Brain, liver, kidney, heart, and spleen tissues of the tumor-bearing mice exhibited decreased malonaldehyde levels. Similar results were obtained for formaldehyde and acetaldehyde.",

author = "Ebeler, {Susan E.} and Hinrichs, {Steven H.} and Clifford, {Andrew J.} and Takayuki Shibamoto",

note = "Funding Information: This research was funded in part by an NIEHS Training Grant and a Nissan scholarship for S.E.E., and by NIH DK 43098 and CA 49624.",

year = "1994",

month = mar,

day = "18",

doi = "10.1016/0378-4347(93)E0436-T",

language = "English (US)",

volume = "654",

pages = "9--18",

journal = "Journal of Chromatography B: Biomedical Sciences and Applications",

issn = "0378-4347",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Volatile carbonyl levels in tissues of transgenic mice with nerve sheath tumors

AU - Ebeler, Susan E.

AU - Hinrichs, Steven H.

AU - Clifford, Andrew J.

AU - Shibamoto, Takayuki

N1 - Funding Information: This research was funded in part by an NIEHS Training Grant and a Nissan scholarship for S.E.E., and by NIH DK 43098 and CA 49624.

PY - 1994/3/18

Y1 - 1994/3/18

N2 - Volatile carbonyl compounds in homogenates prepared from various tissues of tumor-bearing transgenic mice were determined. Formaldehyde and acetaldehyde were derivatized to thiazolidines. Malonaldehyde was derivatized to 1-methylpyrazole. The derivatives were quantified by gas chromatography with a highly sensitive and specific nitrogen-phosphorus detector. The limits of quantitation of formaldehyde and malonaldehyde were 2 μg/ml of homogenate and 27 ng/ml of homogenate, respectively. Levels of malonaldehyde in the erythrocytes and gastrocnemius of tumor-bearing transgenic mice were elevated as compared to the same tissue in control non-transgenic mice. Brain, liver, kidney, heart, and spleen tissues of the tumor-bearing mice exhibited decreased malonaldehyde levels. Similar results were obtained for formaldehyde and acetaldehyde.

AB - Volatile carbonyl compounds in homogenates prepared from various tissues of tumor-bearing transgenic mice were determined. Formaldehyde and acetaldehyde were derivatized to thiazolidines. Malonaldehyde was derivatized to 1-methylpyrazole. The derivatives were quantified by gas chromatography with a highly sensitive and specific nitrogen-phosphorus detector. The limits of quantitation of formaldehyde and malonaldehyde were 2 μg/ml of homogenate and 27 ng/ml of homogenate, respectively. Levels of malonaldehyde in the erythrocytes and gastrocnemius of tumor-bearing transgenic mice were elevated as compared to the same tissue in control non-transgenic mice. Brain, liver, kidney, heart, and spleen tissues of the tumor-bearing mice exhibited decreased malonaldehyde levels. Similar results were obtained for formaldehyde and acetaldehyde.

UR - http://www.scopus.com/inward/record.url?scp=0028293596&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028293596&partnerID=8YFLogxK

U2 - 10.1016/0378-4347(93)E0436-T

DO - 10.1016/0378-4347(93)E0436-T

M3 - Article

C2 - 8004248

AN - SCOPUS:0028293596

SN - 0378-4347

VL - 654

SP - 9

EP - 18

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

IS - 1

ER -

Volatile carbonyl levels in tissues of transgenic mice with nerve sheath tumors

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this