Volatile carbonyl levels in tissues of transgenic mice with nerve sheath tumors

Susan E. Ebeler, Steven H. Hinrichs, Andrew J. Clifford, Takayuki Shibamoto

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Volatile carbonyl compounds in homogenates prepared from various tissues of tumor-bearing transgenic mice were determined. Formaldehyde and acetaldehyde were derivatized to thiazolidines. Malonaldehyde was derivatized to 1-methylpyrazole. The derivatives were quantified by gas chromatography with a highly sensitive and specific nitrogen-phosphorus detector. The limits of quantitation of formaldehyde and malonaldehyde were 2 μg/ml of homogenate and 27 ng/ml of homogenate, respectively. Levels of malonaldehyde in the erythrocytes and gastrocnemius of tumor-bearing transgenic mice were elevated as compared to the same tissue in control non-transgenic mice. Brain, liver, kidney, heart, and spleen tissues of the tumor-bearing mice exhibited decreased malonaldehyde levels. Similar results were obtained for formaldehyde and acetaldehyde.

Original languageEnglish (US)
Pages (from-to)9-18
Number of pages10
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume654
Issue number1
DOIs
StatePublished - Mar 18 1994

ASJC Scopus subject areas

  • Chemistry(all)

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