TY - JOUR
T1 - Water insoluble and soluble lipids for gene delivery
AU - Mahato, Ram I.
N1 - Funding Information:
I would like to acknowledge UT start-up funds, UT Vascular Center of Excellence and Expression Genetics, Inc. for financial support for my lab. I would also like to thank Drs Sung Wan Kim of University of Utah, Mitsuru Hashida of Kyoto University and Alain Rolland of then Valentis, Inc. for their guidance and support while I was working in their laboratories. My sincere thanks also to Kenji Kawabata, Minhyung Lee, Sang-oh Han, Anurag Maheswari, Darin Furgeson, Richard Cohen, Dong-an Wang, Ajit Narang, Neeraj Kumar and Zhaoyang Ye for technical assistance.
PY - 2005/4/5
Y1 - 2005/4/5
N2 - Among various synthetic gene carriers currently in use, liposomes composed of cationic lipids and co-lipids remain the most efficient transfection reagents. Physicochemical properties of lipid/plasmid complexes, such as cationic lipid structure, cationic lipid to co-lipid ratio, charge ratio, particle size and zeta potential have significant influence on gene expression and biodistribution. However, most cationic lipids are toxic and cationic liposomes/plasmid complexes do not disperse well inside the target tissues because of their large particle size. To overcome the problems associated with cationic lipids, we designed water soluble lipopolymers for gene delivery to various cells and tissues. This review provides a critical discussion on how the components of water insoluble and soluble lipids affect their transfection efficiency and biodistribution of lipid/plasmid complexes.
AB - Among various synthetic gene carriers currently in use, liposomes composed of cationic lipids and co-lipids remain the most efficient transfection reagents. Physicochemical properties of lipid/plasmid complexes, such as cationic lipid structure, cationic lipid to co-lipid ratio, charge ratio, particle size and zeta potential have significant influence on gene expression and biodistribution. However, most cationic lipids are toxic and cationic liposomes/plasmid complexes do not disperse well inside the target tissues because of their large particle size. To overcome the problems associated with cationic lipids, we designed water soluble lipopolymers for gene delivery to various cells and tissues. This review provides a critical discussion on how the components of water insoluble and soluble lipids affect their transfection efficiency and biodistribution of lipid/plasmid complexes.
KW - Biodistribution
KW - Cationic liposomes
KW - Gene delivery
KW - Water soluble lipopolymers
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U2 - 10.1016/j.addr.2004.12.005
DO - 10.1016/j.addr.2004.12.005
M3 - Review article
C2 - 15757756
AN - SCOPUS:14744291006
VL - 57
SP - 699
EP - 712
JO - Advanced Drug Delivery Reviews
JF - Advanced Drug Delivery Reviews
SN - 0169-409X
IS - 5
ER -