West African Anopheles gambiae mosquitoes harbor a taxonomically diverse virome including new insect-specific flaviviruses, mononegaviruses, and totiviruses

Joseph R. Fauver, Nathan D. Grubaugh, Benjamin J. Krajacich, James Weger-Lucarelli, Steven M. Lakin, Lawrence S. Fakoli, Fatorma K. Bolay, Joseph W. Diclaro, Kounbobr Roch Dabiré, Brian D. Foy, Doug E. Brackney, Gregory D. Ebel, Mark D. Stenglein

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Anopheles gambiae are a major vector of malaria in sub-Saharan Africa. Viruses that naturally infect these mosquitoes may impact their physiology and ability to transmit pathogens. We therefore used metagenomics sequencing to search for viruses in adult Anopheles mosquitoes collected from Liberia, Senegal, and Burkina Faso. We identified a number of virus and virus-like sequences from mosquito midgut contents, including 14 coding-complete genome segments and 26 partial sequences. The coding-complete sequences define new viruses in the order Mononegavirales, and the families Flaviviridae, and Totiviridae. The identification of a flavivirus infecting Anopheles mosquitoes broadens our understanding of the evolution and host range of this virus family. This study increases our understanding of virus diversity in general, begins to define the virome of a medically important vector in its natural setting, and lays groundwork for future studies examining the potential impact of these viruses on anopheles biology and disease transmission.

Original languageEnglish (US)
Pages (from-to)288-299
Number of pages12
JournalVirology
Volume498
DOIs
StatePublished - Nov 1 2016
Externally publishedYes

Keywords

  • Anopheles gambiae
  • Arthropod viruses
  • Bioinformatics
  • Flavivirus
  • Metagenomics
  • Mononegavirus
  • Pathogen discovery
  • Surveillance
  • Totivirus
  • Virome
  • Virus discovery

ASJC Scopus subject areas

  • Virology

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