Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy-treated macaques

Philip J. Santangelo, Kenneth A. Rogers, Chiara Zurla, Emeline L. Blanchard, Sanjeev Gumber, Karen Strait, Fawn Connor-Stroud, David M. Schuster, Praveen K. Amancha, Jung Joo Hong, Siddappa N. Byrareddy, James A. Hoxie, Brani Vidakovic, Aftab A. Ansari, Eric Hunter, Francois Villinger

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

The detection of viral dynamics and localization in the context of controlled HIV infection remains a challenge and is limited to blood and biopsies. We developed a method to capture total-body simian immunodeficiency virus (SIV) replication using immunoPET (antibody-targeted positron emission tomography). The administration of a poly(ethylene glycol)-modified, 64 Cu-labeled SIV Gp120-specific antibody led to readily detectable signals in the gastrointestinal and respiratory tract, lymphoid tissues and reproductive organs of viremic monkeys. Viral signals were reduced in aviremic antiretroviral-treated monkeys but detectable in colon, select lymph nodes, small bowel, nasal turbinates, the genital tract and lung. In elite controllers, virus was detected primarily in foci in the small bowel, select lymphoid areas and the male reproductive tract, as confirmed by quantitative reverse-transcription PCR (qRT-PCR) and immunohistochemistry. This real-time, in vivo viral imaging method has broad applications to the study of immunodeficiency virus pathogenesis, drug and vaccine development, and the potential for clinical translation.

Original languageEnglish (US)
Pages (from-to)427-432
Number of pages6
JournalNature Methods
Volume12
Issue number5
DOIs
StatePublished - Apr 29 2015

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy-treated macaques'. Together they form a unique fingerprint.

  • Cite this

    Santangelo, P. J., Rogers, K. A., Zurla, C., Blanchard, E. L., Gumber, S., Strait, K., Connor-Stroud, F., Schuster, D. M., Amancha, P. K., Hong, J. J., Byrareddy, S. N., Hoxie, J. A., Vidakovic, B., Ansari, A. A., Hunter, E., & Villinger, F. (2015). Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy-treated macaques. Nature Methods, 12(5), 427-432. https://doi.org/10.1038/nmeth.3320