Wound splinting modulates granulation tissue proliferation

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11 Scopus citations


Attachment of the extracellular matrix to a substratum is important for fibroblast survival and proliferation in three-dimensional in vitro culture systems. We hypothesized that wound matrix attachment in a wound splinting model would modulate wound cell proliferation in vivo. Male rats were excisionally wounded on the dorsum, and a splint was sutured to the wound edge. In one experiment (N=12), 6 rats were desplinted on day 5, and then all were sacrificed 24 h later, 6 h after 5-bromo-2′-deoxyuridine (BrdU) injection. In the second experiment (N=18), 6 rats each were desplinted, desplinted with wound edge release, or not disturbed, followed by BrdU injection and sacrifice 24 h later. BrdU-labeled nuclei were quantified on frozen sections of granulation tissue, cut at three different levels. In the first experiment, the percentage of BrdU-positive nuclei per high power field (hpf) in the splinted vs. desplinted animals was 6.15±2.45 (S.D.) vs. 3.03±1.58%* p<0.001, ANOVA. In the second experiment, the number of BrdU-positive per hpf was 33.1±17.4 vs. 14.5±17.1 vs. 10.2±9.1* (splinted vs. desplinted vs. desplinted/released);*p<0.001 [analysis of variance (ANOVA)]. Removal of the wound splint decreased the rate of BrdU-labeled cells in the granulation tissue by ∼50%; complete disruption of wound matrix attachment may have decreased this rate even further. Wound cell proliferation is modulated by lateral attachment of the wound matrix.

Original languageEnglish (US)
Pages (from-to)243-250
Number of pages8
JournalMatrix Biology
Issue number4
StatePublished - Jul 2004


  • 5-bromo-2′-deoxyuridine
  • BrdU
  • Cell cycle
  • DAB
  • FPCM
  • Granulation tissue
  • PBS
  • Proliferation
  • Rat
  • Wound healing
  • analysis of variance
  • diaminobenzidine
  • dots per inch
  • dpi
  • fibroblast-populated collagen matrix
  • high power field
  • hpf

ASJC Scopus subject areas

  • Molecular Biology


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