DNA polymerase ζ (Pol ζ) is one of the key players in translesion DNA synthesis in eukaryotic cells. In both mammals and lower eukaryotes, Pol ζ is responsible for the generation of the majority of mutations induced by DNA-damaging agents. The mechanisms that regulate the participation of Pol ζ in DNA replication and its contribution to mutagenesis are currently a subject of active investigation. The author's group has found that the function of Pol ζ is not limited to lesion bypass. They have shown that Pol ζ can participate in DNA replication and cause increased mutagenesis in response to a variety of defects in the normal replication machinery. Currently, their research focuses on the mechanisms that license the access of Pol ζ to the primer terminus during DNA replication in the absence of exogenous DNA damage. The researchers have found that proliferating cell nuclear antigen (PCNA), a processivity factor of many nuclear DNA polymerases, plays at least three important and apparently separate roles in regulating Pol ζ-dependent mutagenesis. Initial evidence suggests that the pathways that lead to the recruitment of Pol ζ to the primer terminus, including the role of PCNA, are different in response to DNA damage and intrinsic replisome defects.
|Original language||English (US)|
|Number of pages||11|
|State||Published - Mar 2007|
ASJC Scopus subject areas
- Molecular Biology