Yeast origins establish a strand bias for replicational mutagenesis

Youri I. Pavlov, Carol S. Newlon, Thomas A. Kunkel

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

To determine whether replicational mutagenesis in the yeast genome is influenced by the positions of active origins, a reporter gene was placed in two orientations at multiple locations within a 39,000 bp region of chromosome III possessing two strong origins. The frequency of mutations resulting from misincorporation of adenine opposite 8-hydroxyguanine in one strand and 6-hydroxylaminopurine opposite cytosine in the other strand differed by 3- to 10-fold, depending on the gene orientation and its distance from the origins. The observed patterns indicate that active origins establish a strand bias for mutations that is maintained over thousands of base pairs and results from lower nucleotide selectivity and/or less efficient proofreading or mismatch repair during leading strand DNA replication.

Original languageEnglish (US)
Pages (from-to)207-213
Number of pages7
JournalMolecular Cell
Volume10
Issue number1
DOIs
StatePublished - 2002

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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